Digital images in bioimaging for beginners
Digital images for beginners How does the computer store a microscopy image? Photographs and microscopy images are recordings of real-world objects acquired with imaging devices. A microscopy image is thus a representation of the real object based on experimental setup and the device used (i.e., not "the" reality). The light that was detected during image acquisition is transformed into a digital representation of [...]
Basics of Imaging File Formats
Bioimaging File Formats explained A computer's most basic unit of information is a simple distinction between two states: on or off. This is represented as a binary digit (or bit) with a value of either 1 (on) or 0 (off). To store more complex information, multiple bits are combined. For example, 8 bits together make up a "byte", which is a standard [...]
Bio-Formats – a translator of different file formats
Translating Bioimaging File Formats with "Bio-Formats" The Open Microscopy Environment Consortium has created the so-called Bio-Formats library (Linkert et al., 2010, J Cell Biol) to enable the translation from a large number of proprietary file formats to the OME data model, generating OME-TIFF as a potential output file. Most files generated at the vendor-specific microscopes are written in proprietary, i.e., vendor-owned file formats. Aside from [...]
Next-generation file formats for bioimaging
What are next-generation file formats (NGFF)? Structure of a classical file format As introduced in Bioimaging File Formats explained, microscopy image data can be written in various different file formats, mostly defined by the vendors of the microscopes used. Most of these files and also the "de facto" open community standard file "OME-TIFF" are classical file formats. I.e., these files are written as a series [...]
